Emotional Experiences of Mothers of Newborns Admitted to the NICU

The transition to parenthood is an emotional life experience. Giving birth to a healthy baby and bringing the child home shortly thereafter is a significant emotional occasion. Even when everything goes as smoothly as possible, parents often deal with various degrees of stress and anxiety. Their lives will never be the same and they will have an entire new set of responsibilities swaddled in a little blanket. Some parents do not experience this transition the way they had envisioned and are forced to spend the first phase of their child’s life within the walls of a Neonatal Intensive Care Unit (NICU). Studies have shown that this situation has major emotional implications for parents, both immediate and long-term. The purpose of this study is to describe the self-identified emotional responses of mothers who experienced having a newborn child hospitalized in the NICU immediately following birth.Nursin

Time-lapse cross-hole electrical resistivity tomography (CHERT) for monitoring seawater intrusion dynamics in a Mediterranean aquifer

Surface electrical resistivity tomography (ERT) is a widely used tool to study seawater intrusion (SWI). It is noninvasive and offers a high spatial coverage at a low cost, but its imaging capabilities are strongly affected by decreasing resolution with depth. We conjecture that the use of CHERT (cross-hole ERT) can partly overcome these resolution limitations since the electrodes are placed at depth, which implies that the model resolution does not decrease at the depths of interest. The objective of this study is to test the CHERT for imaging the SWI and monitoring its dynamics at the Argentona site, a well-instrumented field site of a coastal alluvial aquifer located 40¿km NE of Barcelona. To do so, we installed permanent electrodes around boreholes attached to the PVC pipes to perform time-lapse monitoring of the SWI on a transect perpendicular to the coastline. After 2 years of monitoring, we observe variability of SWI at different timescales: (1) natural seasonal variations and aquifer salinization that we attribute to long-term drought and (2) short-term fluctuations due to sea storms or flooding in the nearby stream during heavy rain events. The spatial imaging of bulk electrical conductivity allows us to explain non-monotonic salinity profiles in open boreholes (step-wise profiles really reflect the presence of freshwater at depth). By comparing CHERT results with traditional in situ measurements such as electrical conductivity of water samples and bulk electrical conductivity from induction logs, we conclude that CHERT is a reliable and cost-effective imaging tool for monitoring SWI dynamics.This work was funded by the project CGL2016-77122-C2-1-R/2-R of the Spanish Government. This project also received funding from the European Commission, Horizon 2020 research and innovation programme (Marie Sklodowska-Curie (grant no. 722028)). The author Albert Folch is a Serra Húnter Fellow.Peer ReviewedPostprint (published version

In Vitro and Ectopic In Vivo Studies toward the Utilization of Rapidly Isolated Human Nasal Chondrocytes for Single-Stage Arthroscopic Cartilage Regeneration Therapy

Nasal chondrocytes (NCs) have a higher and more reproducible chondrogenic capacity than articular chondrocytes, and the engineered cartilage tissue they generate in vitro has been demonstrated to be safe in clinical applications. Here, we aimed at determining the feasibility for a single-stage application of NCs for cartilage regeneration under minimally invasive settings. In particular, we assessed whether NCs isolated using a short collagenase digestion protocol retain their potential to proliferate and chondro-differentiate within an injectable, swiftly cross-linked and matrix-metalloproteinase (MMP)-degradable polyethylene glycol (PEG) gel enriched with human platelet lysate (hPL). NC-hPL-PEG gels were additionally tested for their capacity to generate cartilage tissue in vivo and to integrate into cartilage/bone compartments of human osteochondral plugs upon ectopic subcutaneous implantation into nude mice. NCs isolated with a rapid protocol and embedded in PEG gels with hPL at low cell density were capable of efficiently proliferating and of generating tissue rich in glycosaminoglycans and collagen II. NC-hPL-PEG gels developed into hyaline-like cartilage tissues upon ectopic in vivo implantation and integrated with surrounding native cartilage and bone tissues. The delivery of NCs in PEG gels containing hPL is a feasible strategy for cartilage repair and now requires further validation in orthotopic in vivo models. Keywords: cartilage regeneration; autologous chondrocyte implantation; nasal chondrocytes; single-stage; arthroscopy; tissue engineering; polyethylene glycol; hydrogel; platelet lysat

Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple‐negative breast cancer

Cell cycle; Gene expression; Triple-negative breast cancerCiclo celular; Expresión génica; Cáncer de mama triple negativoCicle cel·lular; Expressió gènica; Càncer de mama triple negatiuTriple‐negative breast cancer (TNBC) often develops resistance to single‐agent treatment, which can be circumvented using targeted combinatorial approaches. Here, we demonstrate that the simultaneous inhibition of LOXL2 and BRD4 synergistically limits TNBC proliferation in vitro and in vivo. Mechanistically, LOXL2 interacts in the nucleus with the short isoform of BRD4 (BRD4S), MED1, and the cell cycle transcriptional regulator B‐MyB. These interactions sustain the formation of BRD4 and MED1 nuclear transcriptional foci and control cell cycle progression at the gene expression level. The pharmacological co‐inhibition of LOXL2 and BRD4 reduces BRD4 nuclear foci, BRD4‐MED1 colocalization, and the transcription of cell cycle genes, thus suppressing TNBC cell proliferation. Targeting the interaction between BRD4S and LOXL2 could be a starting point for the development of new anticancer strategies for the treatment of TNBC.We thank the CRG genomics unit, the CRG‐UPF flow cytometry unit, and the VHIO mouse facility for their contribution. We thank Pharmaxis for the supply of PXS LOXL2 inhibitors. SS is supported by the Plan Estatal de I + D + I (COMBAT PID2019‐110598GA‐I00), and the ERC Starting Grant (ERC‐StG‐852343‐EPICAMENTE). LP‐R is supported by the Juan de la Cierva‐Formación fellowship (FJC2019‐040598‐I) and Fundación Franscico Cobos fellowship. TVT is supported by Plan Estatal de I + D + I (PID2019‐108008RJ‐I00), AECC (INVES20036TIAN), and a Ramón y Cajal investigator contract (RYC2020‐029098‐I). DC is supported by the la Caixa Foundation PhD fellowship (ID 100010434; fellowship code LCF/BQ/DI19/11730061)

Time-lapse cross-hole electrical resistivity tomography (CHERT) for monitoring seawater intrusion dynamics in a Mediterranean aquifer

Surface electrical resistivity tomography (ERT) is a widely used tool to study seawater intrusion (SWI). It is noninvasive and offers a high spatial coverage at a low cost, but its imaging capabilities are strongly affected by decreasing resolution with depth. We conjecture that the use of CHERT (cross-hole ERT) can partly overcome these resolution limitations since the electrodes are placed at depth, which implies that the model resolution does not decrease at the depths of interest. The objective of this study is to test the CHERT for imaging the SWI and monitoring its dynamics at the Argentona site, a well-instrumented field site of a coastal alluvial aquifer located 40 km NE of Barcelona. To do so, we installed permanent electrodes around boreholes attached to the PVC pipes to perform time-lapse monitoring of the SWI on a transect perpendicular to the coastline. After 2 years of monitoring, we observe variability of SWI at different timescales: (1) natural seasonal variations and aquifer salinization that we attribute to long-term drought and (2) short-term fluctuations due to sea storms or flooding in the nearby stream during heavy rain events. The spatial imaging of bulk electrical conductivity allows us to explain non-monotonic salinity profiles in open boreholes (step-wise profiles really reflect the presence of freshwater at depth). By comparing CHERT results with traditional in situ measurements such as electrical conductivity of water samples and bulk electrical conductivity from induction logs, we conclude that CHERT is a reliable and cost-effective imaging tool for monitoring SWI dynamics

Oscillations in Cdc14 release and sequestration reveal a circuit underlying mitotic exit

The phosphatase Cdc14 exerts negative feedback on its upstream regulators to limit its release from the nucleolus to once per cell cycle

El compromís de l’ICM amb la igualtat de gènere en la recerca marina

2 pages, 1 figure[EN] Despite the drive towards equality between women and men in research institutions in recent years and the progress that has been made, there is still a long road ahead. Science is still associated with the male image, and women’s work and contributions are often invisible. Like many other spheres of society, research institutions contribute to the structural re(production) of gender inequalities. Women and men each tend to have more presence in certain scientific fields, with the horizontal segregation that this involves. Furthermore, the “glass ceiling” (the invisible barrier that hinders women’s access to the highest levels of decision-making or responsibility, limiting their professional careers) causes vertical segregation. Staying in research is an obstacle course in which there is a constant trickle of skills and talents, which is exacerbated for women. Moreover, research often seems blind to the gender dimension in its approach, content and analysis. […][ES] A pesar del impulso de la igualdad entre mujeres y hombres en las instituciones de investigación en los últimos años y de los avances logrados, aún queda un largo camino por recorrer. La ciencia constituye todavía un ámbito asociado a la imagen masculina, invisibilizando con frecuencia el trabajo y las aportaciones de las mujeres. Como muchas otras esferas de la sociedad, las instituciones de investigación contribuyen a la re(producción) estructural de les desigualdades de género. Mujeres y hombres tienden a concentrarse en determinados campos científicos con la segregación horizontal que ello implica. Por otra parte, el llamado “techo de cristal” –la barrera invisible que dificulta u obstaculiza el acceso de las mujeres a los niveles más altos de poder, de decisión o de responsabilidad, limitando sus carreras profesionales–, materializa la segregación vertical. La permanencia en la investigación es una carrera de obstáculos en la que se produce un goteo constante de capacidades y talentos, que se agrava para las mujeres. Además, la investigación parece a menudo ciega a la importancia de considerar la dimensión de género en su abordaje, contenido y análisis. […][CAT] Malgrat l’impuls envers la igualtat a les institucions de recerca dels darrers anys i els avenços assolits, encara hi ha un llarg camí per recórrer. La ciència constitueix encara un àmbit associat a la imatge masculina, invisibilitzant sovint el treball i les aportacions de les dones. Com moltes altres esferes de la societat, les institucions de recerca contribueixen a la re(producció) estructural de les desigualtats de gènere. Dones i homes tendeixen a concentrar-se en determinats camps científics amb la segregació horitzontal que això implica. Per altra banda, l’anomenat “sostre de vidre” –la barrera invisible que dificulta o obstaculitza l’accés de les dones als nivells més alts de poder, de decisió o de responsabilitat, encotillant les seves carreres professionals–, materialitza la segregació vertical. La permanència en la recerca és una carrera d’obstacles on es produeix un degoteig constant de capacitats i talents, agreujat entre les dones. A més, la recerca sembla sovint cega a la importància de considerar la dimensió de gènere en el seu abordatge, contingut i anàlisi. […]Peer reviewe

Jardins per a la salut

Facultat de Farmàcia, Universitat de Barcelona. Ensenyament: Grau de Farmàcia. Assignatura: Botànica farmacèutica. Curs: 2014-2015. Coordinadors: Joan Simon, Cèsar Blanché i Maria Bosch.Els materials que aquí es presenten són el recull de les fitxes botàniques de 128 espècies presents en el Jardí Ferran Soldevila de l’Edifici Històric de la UB. Els treballs han estat realitzats manera individual per part dels estudiants dels grups M-3 i T-1 de l’assignatura Botànica Farmacèutica durant els mesos de febrer a maig del curs 2014-15 com a resultat final del Projecte d’Innovació Docent «Jardins per a la salut: aprenentatge servei a Botànica farmacèutica» (codi 2014PID-UB/054). Tots els treballs s’han dut a terme a través de la plataforma de GoogleDocs i han estat tutoritzats pels professors de l’assignatura. L’objectiu principal de l’activitat ha estat fomentar l’aprenentatge autònom i col·laboratiu en Botànica farmacèutica. També s’ha pretès motivar els estudiants a través del retorn de part del seu esforç a la societat a través d’una experiència d’Aprenentatge-Servei, deixant disponible finalment el treball dels estudiants per a poder ser consultable a través d’una Web pública amb la possibilitat de poder-ho fer in-situ en el propi jardí mitjançant codis QR amb un smartphone

Colloque "Comparing the Concepts of Representation", Concepta/CivCons/CLAIMS/HiSoPo, Paris, 18-19 novembre 2016

HiSoPo vous informe de la tenue du colloque  "Comparing the Concepts of Representation" organisé par le projet Civic Constellation, le réseau Concepta, le projet ANR-DFG CLAIMS et le groupe de projet de l’AFSP HiSoPo. L'événement aura lieu à Sciences Po & Paris 8, les 18 et 19 novembre 2016. CONCEPTA RESEARCH SEMINAR Comparing the Concepts of Representation SciencesPo/Paris 8, Paris, Friday 18 and Saturday 19 November 2016 Organized by the Civic Constellation II project, Concepta: Internati..

Hyperphysiological compression of articular cartilage induces an osteoarthritic phenotype in a cartilage-on-a-chip model

Owing to population aging, the social impact of osteoarthritis (OA)-the most common musculoskeletal disease-is expected to increase dramatically. Yet, therapy is still limited to palliative treatments or surgical intervention, and disease-modifying OA (DMOA) drugs are scarce, mainly because of the absence of relevant preclinical OA models. Therefore, in vitro models that can reliably predict the efficacy of DMOA drugs are needed. Here, we show, using a newly developed microphysiological cartilage-on-a-chip model that enables the application of strain-controlled compression to three-dimensional articular cartilage microtissue, that a 30% confined compression recapitulates the mechanical factors involved in OA pathogenesis and is sufficient to induce OA traits. Such hyperphysiological compression triggers a shift in cartilage homeostasis towards catabolism and inflammation, hypertrophy, and the acquisition of a gene expression profile akin to those seen in clinical osteoarthritic tissue. The cartilage on-a-chip model may enable the screening of DMOA candidates